Ok, I’ll try to speak without emotion, or at least not in superlatives.
I can’t.
My first real-working-day in the lab. OMG, Amazing! We started at 9, until 11 we discuss the project that contains many steps from the oligonucleotides to multi-colour Fucci cells (yes that is the purpose). If anyone doesn’t know the Fucci cells (which has possibility, who knows it nowadays…), it is a kind of cell that change its colour during cell cycle (szell szájtyl, ahogy a kolléga, aki amúgy nem is phd-s, csak lesz, mondaná). The college who is not even a phd student, told us everything about our project in a quite understandable way. After that we took a break, because he went somewhere and when he came back, we took another break before we went to the robot. I told you about the robot, which worked the whole weekend and has barcode scanner. Now he started it again, its job was to take several pictures about the nuclei of Fucci cells (dyed with dapi). Then, we eat lunch and took a break before we started to work. During lunch Ruslan, one guy from the lab told us that Johan has a great talent. Since that I feel a kind of respect towards him.
After lunch we started to do something interesting. At first, we had to anneal “primers” ordered from a company. I say “primers” since they are not exactly primers as they contain the whole shRNA and the restriction enzyme cleavage site. I was free to mix the ingredients. We put the mixture into the pcr and set the program.
The next task was to cleave the vectors (which we put the shRNA into), and to separate the smaller part from the bigger we need to run them in gel. My first gel electrophoresis, my first cleavage-mixture! I can’t describe my feelings. Such a great lab, such an interesting work and such a research-addicted me! On the top of all these, on the gel-lab (which was as huge as every other with many other people) the radio was on!
The Swedish people usually work from 9 to 5. Thus when we finish our work, 6 o’clock, no one was around us and our really-clever-but-not-phd-student hurried home and let us to go home as well. However it was told that we can arrange our time in the lab. Who knows me enough to know what it means exactly? Of course, I can be there as long as I want! As I see, nothing is so difficult that I cannot learn in 3 weeks, and after that I’ll be free to manage my stay here and make as profitable as I can. Wow.
Let’s go running.
